ELIXIGEN
 
 

Classical Media, Liquid

Description Cat.No. Size Price
DMEM DULBECCO'S MODIFICATION OF EAGLE'S MEDIUM
With a four fold increase in amino acids and vitamins, along with additional nutrients, DMEM provides support for growth of a wide variety of cell lines. Originally used for embryonic mouse and virally infected hamster cells.
DMEM LOW GLUCOSE
     
1000mg/L Glucose
ML-1001S
100ml
 
110mg/L Sodium Pyruvate
ML-1001M
500ml
 
3700mg/L Sodium Bicarbonate
ML-1001L
1000ml
 
without L-Glutamine, add 20ml/L (200mM)

   
Storage:2 to 8

   
1000mg/L Glucose
ML-1002S
100ml
 
110mg/L Sodium Pyruvate
ML-1002M
500ml
 
3700mg/L Sodium Bicarbonate
ML-1002L
1000ml
 
with L-Glutamine
     
Storage:2 to 8
     
25mM HEPES buffer
ML-1003S
100ml
 
1000mg/L Glucose
ML-1003M
500ml
 
110mg/L Sodium Pyruvate
ML-1003L
1000ml
 
3700mg/L Sodium Bicarbonate
     
Storage:2 to 8
     
DMEM HIGH GLUCOSE
     
4500 mg/L Glucose
ML-1004S
100ml
 
without Sodium Pyruvate
ML-1004M
500ml
 
3700mg/L Sodium Bicarbonate
ML-1004L
1000ml  
without L-Glutamine, add 20ml/L (200mM)
     
Storage:2 to 8
     
with L-Glutamine
ML-1005S
100ml
 
4500 mg/L Glucose
ML-1005M
500ml
 
without Sodium Pyruvate
ML-1005L
1000ml
 
3700mg/L Sodium Bicarbonate
     
Storage:2 to 8
     
25mM HEPES buffer
ML-1006S
100ml  
4500mg/L Glucose
ML-1006M
500ml  
without Sodium Pyruvate
ML-1006L
1000ml  
3700mg/L Sodium Bicarbonate
     
without L-Glutamine, add 20ml/L (200mM)
     
Storage:2 to 8
     
IMDM ISCOVE'S MODIFIED DULBECCO'S MEDIUM
A modification of Dulbecco's BME using high glucose and pyruvate, additional amino acids, 25mM HEPES, selenium and other components. Designed for serum free growth of primary hemopoietic cells in culture. Additionally supports the growth of lymphocytes, hybridomas and a varirey of hybrid cells.
ISCOVE'S MODIFIED DULBECCO'S MEDIUM
     
25mM HEPES Buffer
ML-2001S
100ml
 
without alpha- Thioglycerol
ML-2001M
500ml
 
3024 mg/L Sodium Bicarbonate
ML-2001L
1000ml
 
without L-Glutamine, add 20 ml/L(200mM)
     
Storage:2 to 8
     
LEIBOVITZ'S MEDIUM L-15
Designed for growth of established cell lines in CO2 free environment. Requires no sodium bicarbonate; buffering is achieved with galactose substituting for glucose and high levels of both D and DL amino acids.
LEIBOVITZ'S MEDIUM L-15
     
requires no Sodium Bicarbonate
ML-3001S
100ml
 
without L-Glutamine, add 10.3 ml/L (200mM)
ML-3001M
500ml
 
Storage:2 to 8
ML-3001L
1000ml
 
McCOY'S MEDIUM 5A (lwakata and Grace Modification)
A general medium for primary and established cell lines using increased glucose, additional vitamins, high phosphate and peptone.
McCOY'S MEDIUM 5A
     
3000 mg/L Glucose
ML-4001S
100ml
 
2200 mg/L Sodium Bicarbonate
ML-4001M
500ml  
without L-Glutamine, add 7.51 ml/L (200mM)
ML-4001L
1000ml  
Storage:2 to 8
     
MEDIUM 199 (Morgan, Morton, and Parker 1950)
Designed to promote continuous growth of primary chick embryo heart and fibroblast cells without serum. The medium is useful with serum, for growth of a broad range of cells.
MEDIUM 199 1X EARLE'S SALTS

   
2200 mg/L Sodium Bicarbonate
ML-5001S
100ml  
without L-Glutamine, add 3.43 ml/L (200mM)
ML-5001M 500ml  
without Cholesterol, Vitamin A Acetate
ML-5001L
1000ml  
Storage:2 to 8
     
MEDIUM 199 1X EARLE'S SALTS HEPES
     
25 mM HEPES buffer
ML-5002S
100ml  
2200 mg/L Sodium Bicarbonate
ML-5002M
500ml  
without L-Glutamine, add 3.43 ml/L (200mM)
ML-5002L
1000ml  
Storage:2 to 8
     
MEM EAGLE'S MINIMUM ESSENTIAL MEDIUM
An uncomplicated medium useful for a wide range of species and cells.
MEM 1X EARLE'S SALTS
     
without Non-Essential Amino Acids
ML-6001S 100ml  
2200 mg/L Sodium Bicarbonate
ML-6001M
500ml  
without L-Glutamine, add 10 ml/L (200mM)
ML-6001L
1000ml
 
Storage:2 to 8

   
MEM NEAA 1X EARLE'S SALTS
     
without Non-Essential Amino Acids ML-6002S 100ml  
2200 mg/L Sodium Bicarbonate ML-6002M 500ml  
without L-Glutamine, add 10 ml/L (200mM) ML-6002L 1000ml  
Storage:2 to 8      
ALPHA MEM MEDIUM
A Modified Eagle's Minimum Essential Medium supplemented with amino acids, and nucleosides. Designed for growth of a mouse-hamster hybridoma cell line, 8-A (Stanners et al, 1971).
ALPHA MEM EARLE'S SALTS

   
without Nucleosides
ML-7001S
100ml  
2200 mg/L Sodium Bicarbonate
ML-7001M
500ml  
without L-Glutamine, add 10 ml/L (200mM)
ML-7001L 1000ml  
Storage:2 to 8
     
ALPHA MEM EARLE'S SALTS NUCLEOSIDES
     
with Dexyribonucleosides
ML-7002S
100ml  
with Ribonucleosides
ML-7002M
500ml  
2200 mg/L Sodium Bicarbonate
ML-7002L
1000ml  
without L-Glutamine, add 10 ml/L (200mM)
     
Storage:2 to 8
     
RPMI MEDIUM 1640
Designed for growth of human leukemia cells in either monolayers or suspension and useful for a wide variety of suspension and for hybridomas.
RPMI MEDIUM 1640


 
2000 mg/L Sodium Bicarbonate
ML-8001S 100ml  
without L-Glutamine, add 10.3 ml/L (200mM)
ML-8001M
500ml  
Storage:2 to 8
ML-8001L
1000ml  
RPMI MEDIUM 1640
     
with L-Glutamine
ML-8002S
100ml  
2000 mg/L Sodium Bicarbonate
ML-8002M
500ml  
Storage:2 to 8
ML-8002L
1000ml  
RPMI MEDIUM 1640 1X HEPES
     
25 mM HEPES buffer
ML-8003S
100ml  
without L-Glutamine, add 10.3 ml/L (200mM)
ML-8003M
500ml  
Storage:2 to 8
ML-8003L
1000ml  
RPMI MEDIUM 1640 1X HEPES
     
25 mM HEPES buffer
ML-8004S
100ml  
with L-Glutamine
ML-8004M
500ml  
Storage:2 to 8
ML-8004L
1000ml  
DEFICIENT RPMI MEDIUM 1640
     
without Phenol Red
ML-8005S
100ml  
2000 mg/L Sodium Bicarbonate
ML-8005M
500ml  
without L-Glutamine, add 10.3 ml/L (200mM)
ML-8005L
1000ml  
Storage:2 to 8
     
WAYMOUTH'S MEDIUM MB752/1
A chemically defined medium designed for growth of morde L cells. Without serum or peptone. Supplemented with serum, supports growth of many cell types.
WAYMOUTH'S MEDIUM MB752/1 1x
     
without L-Glutamine, add 12 ml/L (200mM)
ML-9001
100ml
 
Storage:2 to 8
     
HAM'S NUTRIENT MIXTURE
Orignally developed for cloning Chinese Hamster Ovary and lung cells. Useful, with serum supplements, for a variety of serum free applications including the growth of myeloma and hybridoma cells.
HAM'S F-10
     
1200mg/L Sodium Bicarbonate
ML-10001S
100ml
 
without L-Glutamine, add 5ml/L (200mM)
ML-10001S
500ml
 
Storage:2 to 8
ML-10001L
1000ml
 
HAM'S F-12
     
1176mg/L Sodium Bicarbonate
ML-10002S
100ml
 
without L-Glutamine, add 5ml/L (200mM)
ML-10002M
500ml  
without Linoleic Acid
ML-10002L
1000ml  
Storage:2 to 8
     
HAM'S F-12/DME HIGH GLUCOSE 1:1Mixtrre
     
3151mg/L Glucose
ML-10003
100ml
 
1200mg/L Sodium Bicarbonate
ML-10003SL
500ml  
without L-Glutamine, add 12.5ml/L (200mM)
ML-10003M
1000ml  
without Linoleic Acid
     
Storage:2 to 8
     
HAM'S F-12K(Kaighn's Nutrient Mixture F-12)
     
without Ascorbic and Linoleic Acids
ML-10004S
100ml
 
2500mg/L Sodium Bicarbonate
ML-10004M
500ml
 
without L-Glutamine, add 10ml/L (200mM)
ML-10004L
1000ml
 
Storage:2 to 8
     
COON'S MEDIUM F-12 (Orignal 1969)
Modification of Ham's F-12 and F-12M Medium for isolation and growth of somatic hybrids. Utilizes increased aminoacids, pyruvate and a modified salt base (Konigsberg's-Hanks' Salts).
COON'S MEDIUM F-12 HIGH ZINC 5% CO2
     
modification of Ham's F-12
ML-11001S
100ml  
1176 mg/L Sodium Bicarbonate
ML-11001M
500ml  
without L-Glutamine, add 10 ml/L (200mM)
ML-11001L
1000ml  
Storage:2 to 8
     
COON'S IMPROVED MEDIUM F-12
     
2500mg/L Sodium Bicarbonate
ML-11002S 100ml  
without L-Glutamine, add 10 ml/L (200mM)
ML-11002M 500ml  
without Linoleic Acid
ML-11002L 1000ml  
Storage:2 to 8
     
All products can be with FCS, FBS, antibiotics and anti-PPLO. If you need special formulation, just contact with us. Our policy is that when you open the medium bottle, the media is ready for your immediate use. Please see our liquid media order form.


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